Additional Information
| Cat. No. | FAFTE-MAXI-GP |
|---|---|
| Manufacturer | MEDIBENA |
| Link to |
Product Catalog, Compact Price List |
| Benefits | • Purity: equity to that obtained by 2x CsCl-gradient centrifugation . • Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials. • Time saving: Complete the process in less than 120 minutes. |
| Description | The Filter Plasmid DNA Extraction Maxi Kit is designed for rapid and efficient extraction of high quality plasmid DNA. With provided filter cartridges the bacteria lysates will be removed without centrifugation. Following a gravity-flow procedure, the plasmid DNA is bound to the resin, and the contaminants can be remove with wash buffer. After using this convenient kit, the purified plasmid DNA is suitable for downstream application such as transfection, in vitro transcription and translation, and all enzymatic modification. Depending on the copy-number of plasmids, either a 50-150ml (high copy number) or a 150-400ml (low-copy plasmid) bacterial suspension could be used in the process. When using the standard protocol, the entire midiprep process can be completed in 120 minutes or less and up to 1000 ug of plasmid can be expected. The purity of purified plasmid DNA is equity to that obtained by 2x CsCl-gradient centrifugation and suitable for all molecular biological applications. |
| Key Features | The purified plasmid DNA can be immediately used in any downstream molecular biology application. • Transfecttion • Microinjection • Sequencing • PCR • Restriction enzyme digestion Operation Format: Gravity-Flow |
| Details | In the process, the modified alkaline lysis method and RNase treatment are used to get cleared cell lysate plasmid DNA in crude lysate has been bound to the column, the contaminants can be washed off with wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in 120minutes without ultracentrifuges and HPLC or other toxic reagents. |
| Notes | Storage Conditions: Plasmid DNA Extraction Kit can be stored at room temperature (15-25 ℃ ). After adding RNase A, PDE Buffer1, should be stored at 4 ℃ and is stable for six months. Other buffers and columns can be stored dry for up to 1 year at room temperature (15-25 ℃ ). |
| Storage Temp | See Notes |
| Shipping Conditions | Ambient |
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