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LipoMag Kit

MEDIBENA Life Science & Diagnostic Solutions

A powerful  & unique transfection kit

This new kit is an association of DreamFect Gold and CombiMag reagents. DreamFectGold formulation is a lipofection reagent achieving high transfection efficiency and high transgene expression level. CombiMag is a Magnetofection reagent: versatile and improving transfection efficiency without toxicity. The LipoMag Kit combines both of the advantages of lipofection and magnetofection transfection technique to transfect all kind of cells with the best efficiency!

  • Highest transfection efficiency

  • Achieve superior transgene expression than any other reagent

  • Universal: cell lines, primary cells and ideal for hard-to-transfect cells

  • For all nucleic acids applications

  • Non toxic & biodegradable

  • Serum compatible

  • Simple, ready-to use and rapid

LipoMag Kit enhances number of transfected cells (GFP+)

Transfection efficiency A549 cells
gfp-a549.gif

LipoMag Kit increases protein production

Comparison with competitor
 photos-gfp-beas-cos.gif

 

BEAS-2B and COS-7 cells were transfected with Reagent L., DreamFectTM Gold or with LipoMag Kit in a 24-well plate using 0.5µg of DNA. For LipoMag Kit: DreamfectTM Gold ratio and CombiMag ratio were fixed respectively at 3μL and 1μL per μg of DNA per well. Reagent L and DreamFectTM Gold were used according to manufacturer instruction. GFP expression was assessed under fluorescent microscope 24H after transfection.

LipoMag Kit improves transfection efficiency in primary cells

Percentage of GFP+ cells comparison with competitor

 

graph-endothelial-gfp.gif

 

Co- and sequential transfection can be performed with LipoMag Kit

 Sequential transfection in HeLa & COS-7

  photo-cotransfection.gif

HeLa and COS 7 cells were first transfected with 0.25 µg of GFP expression plasmid mixed with LipoMag Kit (DreamFect™ Gold: 2 µL/µg DNA for HeLa and 3 µL/µg DNA for COS-7 – 1 µL CombiMag per µg DNA for both cell lines). 1 hour later, cells were sequentially transfected with RFP plasmid under the same conditions. Results were assessed by fluorescence microscopy 24 hours later.

Product listing
 
 
LM80000 LipoMag Starting Kit
  250µL CombiMag + 500µL DreamFect Gold + 1 Super Magnetic Plate
 
LM80250 LipoMag Kit 250
  250µL CombiMag + 500µL DreamFect Gold
 
LM80500 LipoMag Kit 500
  500µL CombiMag + 1mL DreamFect Gold

LipoMag Kit increases protein production

Comparison with competitor
 photos-gfp-beas-cos.gif

BEAS-2B and COS-7 cells were transfected with Reagent L., DreamFectTM Gold or with LipoMag Kit in a 24-well plate using 0.5µg of DNA. For LipoMag Kit: DreamfectTM Gold ratio and CombiMag ratio were fixed respectively at 3μL and 1μL per μg of DNA per well. Reagent L and DreamFectTM Gold were used according to manufacturer instruction. GFP expression was assessed under fluorescent microscope 24H after transfection.


LipoMag Kit improves transfection efficiency in primary cells

Percentage of GFP+ cells comparison with competitor

 

graph-endothelial-gfp.gif

 

Co- and sequential transfection can be performed with LipoMag Kit

 Sequential transfection in HeLa & COS-7

  photo-cotransfection.gif

HeLa and COS 7 cells were first transfected with 0.25 µg of GFP expression plasmid mixed with LipoMag Kit (DreamFect™ Gold: 2 µL/µg DNA for HeLa and 3 µL/µg DNA for COS-7 – 1 µL CombiMag per µg DNA for both cell lines). 1 hour later, cells were sequentially transfected with RFP plasmid under the same conditions. Results were assessed by fluorescence microscopy 24 hours later.

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