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Stability TAG Technology

Stability TAG Technology

Exceptional stability at ambient temperature - Stability TAG Technology

All enzymes produced at Solis BioDyne are exceptionally stable at ambient temperature due to a specific genetic modification - Stability TAG. Stability TAG increases polypeptide self life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself. After being stored at ambient temperature for one month (tested up to +35°C), the enzyme remained fully active.

Stability TAG Technology is now applied in all of Solis BioDynes products providing the client with enzymes, end-point Master Mixes and qPCR Mixes with exceptional stability at ambient temperature.

Tested on a variety of proteins

The stabilizing effect of Stability TAG has been tested on a variety of proteins including Taq DNA Polymerase, hEPO, PCNA, pyrophosphatase and hLIF.

 Stability TAG does not effect the properties of the protein

A number of tests with different Stability TAG attached proteins revealed no affect on protein properties. For example, pluripotency was maintained in ES cells during gene targeting using Stability TAG-hLIF; amplification of DNA using Stability TAG-Taq DNA Polymerase (FIREPol® DNA Polymerase) was performed with the same efficiency and accuracy as with regular Taq.

Storage at +35ºC does not effect enzyme performance

products have been tested for stability at +35°C. Our enzymes FIREPol® and HOT FIREPol® DNA Polymerase exhibited no alteration in performance even after four weeks at +35°C. Also no change in performance was detected between qPCR Mix stored at -20°C and the mix stored at +35°C.

Long term stability at -20ºC

In addition to excellent stability at ambient temperature, enzymes produced at Solis BioDyne also exhibit extraordinarily long shelf life. Even after 18 years, our first batch of FIREPol® DNA Polymerase gives satisfactory results in PCR.

Products with this excellent feature TAG

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  1. SB_04-25-10GP 5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 10 MgCl2)

    5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 10 MgCl2)

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    5 x HOT FIREPolĀ® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). See Technical Documentation to get pdf maual. Learn More
  2. SB_04-25-12GP 5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 12,5 MgCl2)

    5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 12,5 MgCl2)

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    5 x HOT FIREPolĀ® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). See Technical Documentation to get pdf maual. Learn More
  3. SB_04-25-7GP 5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 7,5 MgCl2)

    5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 7,5 MgCl2)

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    5 x HOT FIREPolĀ® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). See Technical Documentation to get pdf maual. Learn More
  4. SB_08-26-GP 5x HOT FIREPol EvaGreenĀ® qPCR Mix (Capillary)

    5x HOT FIREPol EvaGreenĀ® qPCR Mix (Capillary)

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    HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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  5. SB_04-33-GP 5x HOT FIREPolĀ® GC Master Mix TESTING SAMPLE

    5x HOT FIREPol GC Master Mix

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    5x HOT FIREPol GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials. Generated PCR products are compatible with TA cloning procedures Master Mix for Highly specific high-yield amplification of GC-rich templates. Compatible with TA cloning. Learn More
  6. SB_08-25-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus (no ROX)

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus (no ROX)

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    HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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  7. SB_08-24-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus with ROX

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus with ROX

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    HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye.

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  8. SB_08-36-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix

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    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix is an optimised ready-to-use solution for real time quantitative PCR assays, incorporating EvaGreenĀ® dye. Suitable for ROX-dependent and ROX-independent qPCR cyclers Learn More
  9. SB_08-17-GP 5x HOT FIREPolĀ® Probe Universal qPCR Mix

    5x HOT FIREPolĀ® Probe Universal qPCR Mix

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    Suitable for ROX-dependent and ROX-independent qPCR cyclers Learn More
  10. SB_01-01-GP FIREPolĀ® DNA Polymerase (5U/ul)

    FIREPolĀ® DNA Polymerase (5U/ul)

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    FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

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MEDIBENA Life Science & Diagnostic Solutions | Zeillergasse 38 (Office: Paschinggasse 8), 1170 Vienna, Austria, Europe | office@medibena.at | TEL: +43-199-064-97 | FAX: +43-1-2533-0339-577 | Skype: medibena | Register of companies: Handelsgericht Wien Ɩsterreich | FN: 330781v | VAT: ATU65121744 | EORI: ATEOS1000017901 | Medibena Dr. Bencur Peter E.U. | www.MEDIBENA.com