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Stability TAG Technology

Stability TAG Technology

Exceptional stability at ambient temperature - Stability TAG Technology

All enzymes produced at Solis BioDyne are exceptionally stable at ambient temperature due to a specific genetic modification - Stability TAG. Stability TAG increases polypeptide self life and its tolerance for temperature at a wider scale without compromising the properties of the polypeptide itself. After being stored at ambient temperature for one month (tested up to +35°C), the enzyme remained fully active.

Stability TAG Technology is now applied in all of Solis BioDynes products providing the client with enzymes, end-point Master Mixes and qPCR Mixes with exceptional stability at ambient temperature.

Tested on a variety of proteins

The stabilizing effect of Stability TAG has been tested on a variety of proteins including Taq DNA Polymerase, hEPO, PCNA, pyrophosphatase and hLIF.

 Stability TAG does not effect the properties of the protein

A number of tests with different Stability TAG attached proteins revealed no affect on protein properties. For example, pluripotency was maintained in ES cells during gene targeting using Stability TAG-hLIF; amplification of DNA using Stability TAG-Taq DNA Polymerase (FIREPol® DNA Polymerase) was performed with the same efficiency and accuracy as with regular Taq.

Storage at +35ºC does not effect enzyme performance

products have been tested for stability at +35°C. Our enzymes FIREPol® and HOT FIREPol® DNA Polymerase exhibited no alteration in performance even after four weeks at +35°C. Also no change in performance was detected between qPCR Mix stored at -20°C and the mix stored at +35°C.

Long term stability at -20ºC

In addition to excellent stability at ambient temperature, enzymes produced at Solis BioDyne also exhibit extraordinarily long shelf life. Even after 18 years, our first batch of FIREPol® DNA Polymerase gives satisfactory results in PCR.

Products with this excellent feature TAG

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  1. SB_28-41-GP  SolisFASTā„¢ SolisGreenĀ® qPCR Mix (No ROX)

    SolisFASTā„¢ SolisGreenĀ® qPCR Mix (No ROX)

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    Fast and highly sensitive dye-based qPCR Learn More
  2. SB_28-46-GP  SolisFASTā„¢ SolisGreenĀ® qPCR Mix (ROX)

    SolisFASTā„¢ SolisGreenĀ® qPCR Mix (ROX)

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    Fast and highly sensitive dye-based qPCR Learn More
  3. SB_05-16-GP 10x GC-rich Enhancer (Additive for PCR reaction)

    10x GC-rich Enhancer (Additive for PCR reaction)

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    10x GC - rich Enhancer is used as PCR additive for difficult GC - rich templates. The optimized solution modifies melting behavio u r of nucleic acids and often enhances amplification of suboptimal PCR systems with high degree of seco ndary structures and GC - rich regions. 10x GC - rich Enhancer should be used at a defined working concentration (1x, 2x or 3x solution) and only if non - specific amplifi cation occurs. Learn More
  4. SB_04-25-10GP 5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 10 MgCl2)

    5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 10 MgCl2)

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    5 x HOT FIREPolĀ® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). See Technical Documentation to get pdf maual. Learn More
  5. SB_04-25-12GP 5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 12,5 MgCl2)

    5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 12,5 MgCl2)

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    5 x HOT FIREPolĀ® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). See Technical Documentation to get pdf maual. Learn More
  6. SB_04-25-7GP 5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 7,5 MgCl2)

    5 x HOT FIREPolĀ® Blend Master Mix (Ready to Load 7,5 MgCl2)

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    5 x HOT FIREPolĀ® Blend Master Mix Ready to Load is a premixed ready-to-use solution containing all reagents required for PCR (except template, primers and water). See Technical Documentation to get pdf maual. Learn More
  7. SB_08-26-GP 5x HOT FIREPol EvaGreenĀ® qPCR Mix (Capillary)

    5x HOT FIREPol EvaGreenĀ® qPCR Mix (Capillary)

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    HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

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  8. SB_04-33-GP 5x HOT FIREPolĀ® GC Master Mix TESTING SAMPLE

    5x HOT FIREPol GC Master Mix

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    5x HOT FIREPol GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials. Generated PCR products are compatible with TA cloning procedures Master Mix for Highly specific high-yield amplification of GC-rich templates. Compatible with TA cloning. Learn More
  9. SB_08-25-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus (no ROX)

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus (no ROX)

    Call or Email for Price

    HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

    Learn More
  10. SB_08-24-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus with ROX

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus with ROX

    Call or Email for Price

    HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye.

    Learn More

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MEDIBENA Life Science & Diagnostic Solutions | Zeillergasse 38, 1170 Vienna, Austria, Europe | office@medibena.at | TEL: +43-699-1133-0065 | FAX: +43-1-2533-0339-577 | Skype: medibena | Register of companies: Handelsgericht Wien Ɩsterreich | FN: 392173a | VAT: ATU67954225 | EORI: ATEOS1000086649 | Dr. Monika Bencurova MEDIBENA Life Science E.U. | www.MEDIBENA.at