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SUPERMIX

qPCR Mix based on Ambient Temperature stable FirePol Hot-start Polymerase

5x HOT FIREPol® EvaGreen® qPCR Supermix is an 5x master mix for ease of use quantification of cDNA, genomic DNA or plasmid DNA, incorporating EvaGreen® dye. It comprises all the components necessary, scientist need to add only template and primers, to perform highly sensitive qPCR. HOTFIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. The hot-start mechanism prevents the extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Benefits

  • Wide instrument compatibility - suitable for qPCR cyclers regardless of ROX requirements (except capillary) 
  • Reaction set-up at room temperature Stability TAG!
  • Highly specific and reproducible real time PCR 
  • Excellent efficiency in case of low copy number targets 
  • UNG treatment capability due to dNTP blend of dUTP/dTTP (Add UNG treatment step ONLY if UNG enzyme is added in the reaction mix)
  • Superior performance with long (up to 500 bp) and GC-rich templates (GC-enhancer)
  • Blue visualisation dye for easy pipetting
  • Internal reference based on ROX dye

        

Applications

  • Detection and quantification of DNA and cDNA
  • Profiling gene expression 
  • Microbial detection 
  • Viral load determination

5 Item(s)

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  1. SB_28-41-GP SolisFASTā„¢ SolisGreenĀ® qPCR Mix (No ROX)

    SolisFASTā„¢ SolisGreenĀ® qPCR Mix (No ROX)

    Call or Email for Price

    Fast and highly sensitive dye-based qPCR Learn More
  2. SB_28-46-GP SolisFASTā„¢ SolisGreenĀ® qPCR Mix (ROX)

    SolisFASTā„¢ SolisGreenĀ® qPCR Mix (ROX)

    Call or Email for Price

    Fast and highly sensitive dye-based qPCR Learn More
  3. SB_08-36-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix

    Call or Email for Price

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix is an optimised ready-to-use solution for real time quantitative PCR assays, incorporating EvaGreenĀ® dye. Suitable for ROX-dependent and ROX-independent qPCR cyclers Learn More
  4. SB_08-46-GP 5x HOT FIREPolĀ® SolisGreen qPCR Mix

    5x HOT FIREPolĀ® SolisGreen qPCR Mix

    Call or Email for Price

    HOT FIREPolĀ® SolisGreen qPCR Mix is an optimised ready-to-use solution for real-time quantitative PCR assays Learn More
  5. SB_06-35-GP SOLIScript RT cDNA synthesis

    SOLIScript RT cDNA synthesis

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    SOLIScript Reverse Transcriptase (RT) is an (in silico) designed chimeric RNAā€directed DNA polymerase Learn More

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