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Probe Based Approach

5 x HOT FIREPol® Probe qPCR Mix Plus

MEDIBENA Life Science & Diagnostic Solutions

5 x HOT FIREPol® Probe qPCR Mix Plus

With
ROX

Applied Biosystems 7900HT, 7500, 7700, 7000, StepOne™ & StepOnePlus™ Stratagene  Mx3000P™ & Mx3005P™

     Without
ROX

 Bio-Rad CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®; Qiagen  Rotor-Gene® Q, Rotor-Gene® 6000 Eppendorf Mastercycler® ep realplex2 & ep realplex4

Tab. 01. Mix compatibility table (Probe based approach)

FREE SAMPLES Solis BioDyne Product line Catalog  -


   

Description

HOT FIREPol® Probe qPCR Mix Plus is an optimized ready-to-use solution for real-time quantitative PCR assays with all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and ROX dye according to system requirements. The user simply needs to add water, template, probe and primers. HOT FIREPol® Probe qPCR Mix Plus is compatible for use with most real-time thermal cyclers. HOT FIREPol® DNA Polymerase is inactive at ambient temperatures and therefore requires an incubation step at 95°C for 15 minutes prior to qPCR. This prevents the extension of nonspecifically annealed primers and primer-dimers being formed at low temperatures, during qPCR set-up.

Features

• High sensitivity with broad dynamic range
• Unique stability at room temperature
• Convenient ready-to-use solution
• Reaction set-up without using ice
• Available with ROX reference dye and without ROX
• Mixes compatible with most real-time thermal cyclers

Applications

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Genotyping
• Microbial detection
• Viral load determination

Quality control

• Functional quality control via real-time PCR on different genomic templates
• Tests for stability at room temperature
• Tests for multiple freeze-thaw cycles

 

As part of our Molecular Epidemiological Research, we find the HOT FIREPol® qPCR Probe Mix very useful in testing common variants such MTHFR C677T, and coagulation factors FII and FVLeiden. The results are remarkably consistent and robust. Must Try!
Dr. Joseph Borg, University of Malta
_____

Performance Data of Probe Mix

Highly competitive

Three tenfold dilutions of 72 bp fragment of Albumin gene were amplified from human genomic DNA using HOT FIREPol® Probe qPCR Mix Plus (red) and a qPCR mix from Company A (green). Reactions were performed on Applied Biosystems 7900HT Real-Time PCR System following cycling protocols recommended by the suppliers.

Excellent reproducibility

Twenty independent amplifications of each dilution show high uniformity and reproducibility of HOT FIREPol® Probe qPCR Mix Plus. Amplification was performed on human genomic DNA using Applied Biosystems 7900HT Real-Time PCR System following cycling protocols recommended by the suppliers.

Multiplex compatible

Amplification of FAM labelled target SNAI1 (orange) and VIC labelled reference gene HPRT (yellow) was performed in a single reaction using HOT FIREPol® Probe qPCR Mix Plus. This multiplex qPCR was carried out on three tenfold dilutions of human placental cDNA on Applied Biosystems 7900HT Real-Time PCR System.

     

 

PRODUCT CONTENTS (PDF) CAT. NO. REACTIONS
5 x HOT FIREPol® Probe
qPCR Mix Plus (ROX)
HOT FIREPol® DNA Polymerase,
5 x Probe qPCR buffer,
15 mM MgCl2, dNTPs, ROX dye
SB_08-14-0000S
SB_08-14-00001
SB_08-14-00020
50
250
5000
5 x HOT FIREPol® Probe
qPCR Mix (no ROX)
HOT FIREPol® DNA Polymerase,
5 x Probe qPCR buffer,
15 mM MgCl2, dNTPs
SB_08-15-0000S
SB_08-15-00001
SB_08-15-00020
50
250
5000

Tab. 02. Product table

2 Item(s)

per page

Set Descending Direction
  1. SB_08-15-GP HOT FIREPol® Probe qPCR Mix Plus (no ROX)

    HOT FIREPol® Probe qPCR Mix Plus (no ROX)

    Call or Email for Price

    HOT FIREPol®Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

    Learn More
  2. sb_08-14-GP HOT FIREPol® Probe qPCR Mix Plus (ROX)

    HOT FIREPol® Probe qPCR Mix Plus (ROX)

    Call or Email for Price

    HOT FIREPol®Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

    Learn More

2 Item(s)

per page

Set Descending Direction
MEDIBENA Life Science & Diagnostic Solutions | Zeillergasse 38, 1170 Vienna, Austria, Europe | office@medibena.at | TEL: +43-699-1133-0065 | FAX: +43-1-2533-0339-577 | Skype: medibena | Register of companies: Handelsgericht Wien Österreich | FN: 392173a | VAT: ATU67954225 | EORI: ATEOS1000086649 | Dr. Monika Bencurova MEDIBENA Life Science E.U. | www.MEDIBENA.at