Probe Based Approach
5 x HOT FIREPol® Probe qPCR Mix Plus
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With |
Applied Biosystems 7900HT, 7500, 7700, 7000, StepOne™ & StepOnePlus™ Stratagene Mx3000P™ & Mx3005P™ |
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Without |
Bio-Rad CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®; Qiagen Rotor-Gene® Q, Rotor-Gene® 6000 Eppendorf Mastercycler® ep realplex2 & ep realplex4 |
Tab. 01. Mix compatibility table (Probe based approach)
FREE SAMPLES Solis BioDyne Product line Catalog -
DescriptionHOT FIREPol® Probe qPCR Mix Plus is an optimized ready-to-use solution for real-time quantitative PCR assays with all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and ROX dye according to system requirements. The user simply needs to add water, template, probe and primers. HOT FIREPol® Probe qPCR Mix Plus is compatible for use with most real-time thermal cyclers. HOT FIREPol® DNA Polymerase is inactive at ambient temperatures and therefore requires an incubation step at 95°C for 15 minutes prior to qPCR. This prevents the extension of nonspecifically annealed primers and primer-dimers being formed at low temperatures, during qPCR set-up. Features• High sensitivity with broad dynamic range Applications• Detection and quantification of DNA and cDNA targets Quality control• Functional quality control via real-time PCR on different genomic templates As part of our Molecular Epidemiological Research, we find the HOT FIREPol® qPCR Probe Mix very useful in testing common variants such MTHFR C677T, and coagulation factors FII and FVLeiden. The results are remarkably consistent and robust. Must Try! Dr. Joseph Borg, University of Malta |
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Performance Data of Probe Mix
Highly competitiveThree tenfold dilutions of 72 bp fragment of Albumin gene were amplified from human genomic DNA using HOT FIREPol® Probe qPCR Mix Plus (red) and a qPCR mix from Company A (green). Reactions were performed on Applied Biosystems 7900HT Real-Time PCR System following cycling protocols recommended by the suppliers.
Excellent reproducibilityTwenty independent amplifications of each dilution show high uniformity and reproducibility of HOT FIREPol® Probe qPCR Mix Plus. Amplification was performed on human genomic DNA using Applied Biosystems 7900HT Real-Time PCR System following cycling protocols recommended by the suppliers.
Multiplex compatibleAmplification of FAM labelled target SNAI1 (orange) and VIC labelled reference gene HPRT (yellow) was performed in a single reaction using HOT FIREPol® Probe qPCR Mix Plus. This multiplex qPCR was carried out on three tenfold dilutions of human placental cDNA on Applied Biosystems 7900HT Real-Time PCR System. |
| PRODUCT | CONTENTS (PDF) | CAT. NO. | REACTIONS |
| 5 x HOT FIREPol® Probe qPCR Mix Plus (ROX) |
HOT FIREPol® DNA Polymerase, 5 x Probe qPCR buffer, 15 mM MgCl2, dNTPs, ROX dye |
SB_08-14-0000S SB_08-14-00001 SB_08-14-00020 |
50 250 5000 |
| 5 x HOT FIREPol® Probe qPCR Mix (no ROX) |
HOT FIREPol® DNA Polymerase, 5 x Probe qPCR buffer, 15 mM MgCl2, dNTPs |
SB_08-15-0000S SB_08-15-00001 SB_08-15-00020 |
50 250 5000 |
Tab. 02. Product table
2 Item(s)
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SB_08-15-GP
HOT FIREPol® Probe qPCR Mix Plus (no ROX)
Call or Email for PriceHOT FIREPol®Probe qPCR Mix Plus (no ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Learn More -
sb_08-14-GP
HOT FIREPol® Probe qPCR Mix Plus (ROX)
Call or Email for PriceHOT FIREPol®Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Learn More
2 Item(s)