MEDIBENA Life Science & Diagnostic Solutions | office@medibena.at | TEL: +43-1-9906-497 | FAX: +43-1-2533-0339-577
call us

Free Shipping on orders over 250€

Order your preferred reagents from Medibena online shop or per E-mail: order@medibena.com

Intercalating-Dye Approach

5 x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus

MEDIBENA Life Science & Diagnostic Solutions

5 x HOT FIREPol® EvaGreen® qPCR Mix Plus

ROX

 Applied Biosystems 7900HT, 7500, 7700, 7000, StepOne™ & StepOnePlus™ Stratagene  Mx3000P™ & Mx3005P™

No ROX

Bio-Rad CFX96™ & CFX384™, iQ™5 & MyiQ™, Chromo4™, Opticon® 2 & MiniOpticon®; Qiagen  Rotor-Gene® Q, Rotor-Gene® 6000 Eppendorf Mastercycler® ep realplex2 & ep realplex4

Tab. 01. Mix compatibility table (Intercalating dye based approach)

FREE SAMPLES Solis BioDyne Product line Catalog  -


   

Description

HOT FIREPol® EvaGreen® qPCR Mix Plus is an optimized ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and ROX dye according to system requirements. The user simply needs to add water, template and primers. EvaGreen® has several features such as high sensitivity, low PCR inhibition and room temperature stability that set it apart from other DNA-binding dyes used for quantitative real-time PCR applications. HOT FIREPol® DNA Polymerase is inactive at ambient temperatures and therefore requires an incubation step at 95°C for 15 minutes prior to qPCR. This prevents the extension of mis-primed products and primer-dimers formed at low temperatures during PCR setup.

EvaGreen® dye

Highly sensitive – produces the most robust PCR signal when used at the recommended concentration
Low PCR inhibition – exhibits much less PCR inhibition than other detection dyes via a smart "release on demand" DNA-binding technology
Extremely stable – simply indestructible under most biochemical conditions. Can be stored at room temperature and be subject to repeated freeze-thaw cycles
Spectrally similar to other popular dyes – compatible with all major brand real-time thermal cyclers
Nonmutagenic and noncytotoxic

Features

• High sensitivity with broad dynamic range
• Unique stability at room temperature
• Based on EvaGreen® dye
• Convenient ready-to-use solution
• Reaction set-up without using ice
• Available with ROX reference dye and without ROX
• Mixes compatible with most real-time thermal cyclers

Applications

• Detection and quantification of DNA and cDNA targets
• Profiling gene expression
• Genotyping
• Microbial detection
• Viral load determination
• High Resolution Melt Analysis

Quality control

• Functional quality control via real-time PCR on different genomic templates
• Tests for stability at room temperature
• Tests for multiple freeze-thaw cycles

 

I regularly use your EvaGreen® qPCR Mix in gene expression assays. It is great value for money and, more importantly, works well allowing good detection sensitivity.
Dr. Aaron Jeffries, King's College London, Institute of Psychiatry 
_____

Performance data of EvaGreen® Mix

 Excellent sensitivity and specificity

The amplification of 98 bp fragment of GAPDH gene exhibits sensitive and efficient reaction curves (upper graph) with highly specific peak in melt curve analysis (lower graph) using HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX). Amplification was performed on human genomic DNA using Rotor-Gene® 6000 qPCR cycler following cycling protocols recommended by the suppliers.

High reproducibility on various qPCR cyclers

Twenty independent amplifications of each dilution show high uniformity and reproducibility on different qPCR platforms. The reactions were carried out on Corbett Rotor-Gene® 6000 (upper graph) using HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) and Roche LightCycler 1.5 (lower graph) using HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary).


   

 

PRODUCT CONTENTS (PDF)
CAT. NO. REACTIONS
     
5 x HOT FIREPol® EvaGreen®
qPCR Mix Plus (ROX)
HOT FIREPol® DNA Polymerase,
5 x EvaGreen® qPCR buffer,
12.5 mM MgCl2, dNTPs, ROX dye
08-24-0000S
08-24-00001
08-24-00020
50
250
5000
5 x HOT FIREPol® EvaGreen®
qPCR Mix Plus (no ROX)
HOT FIREPol® DNA Polymerase,
5 x EvaGreen® qPCR buffer, dNTPs,
12.5 mM MgCl2

08-25-0000S
08-25-00001
08-25-00020
50
250
5000
5 x HOT FIREPol® EvaGreen®
qPCR Mix Plus (Capillary)
HOT FIREPol® DNA Polymerase,
5 x EvaGreen® qPCR buffer, dNTPs,
12.5 mM MgCl2, BSA

08-26-0000S
08-26-00001
08-26-00020
 50
250
5000

Tab. 02. Product detail table

References

Acetylation of H3 K56 is Required for RNA Polymerase II Transcript Elongation through Heterochromatin in Yeast, Varv S, Kristjuhan K, Peil K, Looke M, Mahlakoiv T, Paapsi K, Kristjuhan A Mol Cell Biol. 2010 Jan 11. [Epub ahead of print]

Human TRB3 is upregulated in stressed cells by the induction of translationally efficient mRNA containing a truncated 5'-UTR, Örd T, Ord D, Kõivomägi M, Juhkam K, Örd T. Gene. 2009 Sep 1;444(1-2):24-32.

MEDIBENA Life Science & Diagnostic Solutions

 


<h1 style="text-align: left;">Mix compatibility table</h1>
<p>&nbsp;</p>
<table style="border: 1px solid #669000;" border="1" width="600" rules="all" height="267">
<tbody>
<tr>
<td>
<p style="text-align: center;">qPCR platforms</p>
</td>
<td>
<p style="text-align: center;">Probe based<br />approach</p>
</td>
<td>
<p style="text-align: center;">Intercalating dye based<br />approach</p>
</td>
</tr>
<tr>
<td style="text-align: center;"><strong>Applied Biosystems</strong> 7900HT, 7500, 7700, 7000, <br />StepOne&trade; &amp; StepOnePlus&trade; <strong><br />Stratagene &nbsp;</strong>Mx3000P&trade; &amp; Mx3005P&trade;</td>
<td>
<p style="text-align: center;"><a href="http://www.medibena.at/catalogsearch/result/?q=5+x+HOT+FIREPol%C2%AE+Probe+qPCR+Mix+Plus+%28ROX%29">5 x HOT FIREPol&reg; <br />Probe qPCR&nbsp;&nbsp; <br />Mix Plus (ROX)</a></p>
</td>
<td>
<p style="text-align: center;"><a href="http://www.medibena.at/catalogsearch/result/?q=5+x+HOT+FIREPol%C2%AE+EvaGreen%C2%AE+qPCR+Mix+Plus+%28ROX%29">5 x HOT FIREPol&reg; <br />EvaGreen&reg;<br />qPCR Mix Plus (ROX)</a></p>
</td>
</tr>
<tr>
<td style="text-align: center;"><strong>Bio-Rad </strong>CFX96&trade; &amp; CFX384&trade;, iQ&trade;5 &amp; MyiQ&trade;, <br />Chromo4&trade;, Opticon&reg; 2 &amp; MiniOpticon&reg;; <br /><strong>Qiagen </strong>Rotor-Gene&reg; Q, Rotor-Gene&reg; 6000 <br /> <strong>Eppendorf</strong> Mastercycler&reg; <em>ep realplex</em><sup>2</sup> &amp; <em>ep realplex</em><sup>4</sup></td>
<td>
<p style="text-align: center;"><a href="http://www.medibena.at/catalogsearch/result/?q=5+x+HOT+FIREPol%C2%AE+Probe+qPCR++Mix+Plus+%28no+ROX%29">5 x HOT FIREPol&reg; <br />Probe qPCR<br />Mix Plus (no ROX)</a></p>
</td>
</tr>
</tbody>
</table>

6 Item(s)

per page

Set Descending Direction
  1. SB_08-26-GP 5x HOT FIREPol EvaGreenĀ® qPCR Mix (Capillary)

    5x HOT FIREPol EvaGreenĀ® qPCR Mix (Capillary)

    Call or Email for Price

    HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

    Learn More
  2. SB_04-33-GP 5x HOT FIREPolĀ® GC Master Mix TESTING SAMPLE

    5x HOT FIREPol GC Master Mix

    Call or Email for Price

    5x HOT FIREPol GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials. Generated PCR products are compatible with TA cloning procedures Master Mix for Highly specific high-yield amplification of GC-rich templates. Compatible with TA cloning. Learn More
  3. SB_08-25-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus (no ROX)

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus (no ROX)

    Call or Email for Price

    HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

    Learn More
  4. SB_08-24-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus with ROX

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus with ROX

    Call or Email for Price

    HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye.

    Learn More
  5. SB_08-36-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix

    Call or Email for Price

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix is an optimised ready-to-use solution for real time quantitative PCR assays, incorporating EvaGreenĀ® dye. Suitable for ROX-dependent and ROX-independent qPCR cyclers Learn More
  6. SB_08-17-GP 5x HOT FIREPolĀ® Probe Universal qPCR Mix

    5x HOT FIREPolĀ® Probe Universal qPCR Mix

    Call or Email for Price

    Suitable for ROX-dependent and ROX-independent qPCR cyclers Learn More

6 Item(s)

per page

Set Descending Direction
MEDIBENA Life Science & Diagnostic Solutions | Zeillergasse 38 (Office: Paschinggasse 8), 1170 Vienna, Austria, Europe | office@medibena.at | TEL: +43-199-064-97 | FAX: +43-1-2533-0339-577 | Skype: medibena | Register of companies: Handelsgericht Wien Ɩsterreich | FN: 330781v | VAT: ATU65121744 | EORI: ATEOS1000017901 | Medibena Dr. Bencur Peter E.U. | www.MEDIBENA.com