High-Resolution Melting (HRM)
5 x HOT FIREPol® EvaGreen® HRM Mix
Applied Biosystems 7900HT, 7500, 7700 |
|
Bio-Rad CFX384™, CFX96™ (software update Jun 2010) ,Qiagen Rotor-Gene® Q, Rotor-Gene® 6000 |
Tab. 01. Mix compatibility table (Intercalating dye based approach)
FREE SAMPLES Solis BioDyne Product line Catalog -
|
||
DescriptionHOT FIREPol® EvaGreen® HRM Mix is an optimized ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It includes: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and ROX dye according to system requirements. Features• Sensitive HRM genotyping I am very satisfied with the results of the RT-qPCR I made with the 5 × HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX). It's sensitive to low amount of template and works well with Stratagene Mx3005P™ thermocycler I use. Saad Nizar, PhD Student, University of Strasbourg, Institut de Biologie, Moleculaire et Cellulaire |
_____ |
Performance Data of HRM MixSensitive HRM genotypingHigh Resolution Melt analysis was used to genotype a C insertion in BRCA1 gene, a breast cancer susceptibility gene, with HOT FIREPol® EvaGreen® HRM Mix (two graphs above). Reactions were performed on Corbett Rotor-Gene® 6000. Green lines represent wildtypes without an insertion, red lines represent a C insertion and blue line represents a patient with unknown phenotype. Applications• Detection and quantification of DNA and cDNA targets Quality control• Functional quality control via real-time PCR on different genomic templates |
Tab. 02. Product table
<p> </p>
<table style="border: 1px solid #669000;" border="1" width="600" rules="all" height="267">
<tbody>
<tr>
<td>
<p style="text-align: center;">qPCR platforms</p>
</td>
<td>
<p style="text-align: center;">Probe based<br />approach</p>
</td>
<td>
<p style="text-align: center;">Intercalating dye based<br />approach</p>
</td>
</tr>
<tr>
<td style="text-align: center;"><strong>Applied Biosystems</strong> 7900HT, 7500, 7700, 7000, <br />StepOne™ & StepOnePlus™ <strong><br />Stratagene </strong>Mx3000P™ & Mx3005P™</td>
<td>
<p style="text-align: center;"><a href="http://www.medibena.at/catalogsearch/result/?q=5+x+HOT+FIREPol%C2%AE+Probe+qPCR+Mix+Plus+%28ROX%29">5 x HOT FIREPol® <br />Probe qPCR <br />Mix Plus (ROX)</a></p>
</td>
<td>
<p style="text-align: center;"><a href="http://www.medibena.at/catalogsearch/result/?q=5+x+HOT+FIREPol%C2%AE+EvaGreen%C2%AE+qPCR+Mix+Plus+%28ROX%29">5 x HOT FIREPol® <br />EvaGreen®<br />qPCR Mix Plus (ROX)</a></p>
</td>
</tr>
<tr>
<td style="text-align: center;"><strong>Bio-Rad </strong>CFX96™ & CFX384™, iQ™5 & MyiQ™, <br />Chromo4™, Opticon® 2 & MiniOpticon®; <br /><strong>Qiagen </strong>Rotor-Gene® Q, Rotor-Gene® 6000 <br /> <strong>Eppendorf</strong> Mastercycler® <em>ep realplex</em><sup>2</sup> & <em>ep realplex</em><sup>4</sup></td>
<td>
<p style="text-align: center;"><a href="http://www.medibena.at/catalogsearch/result/?q=5+x+HOT+FIREPol%C2%AE+Probe+qPCR++Mix+Plus+%28no+ROX%29">5 x HOT FIREPol® <br />Probe qPCR<br />Mix Plus (no ROX)</a></p>
</td>
</tr>
</tbody>
</table>
-
SB_08-31-GP
HOT FIREPol EvaGreen® HRM Mix (NO ROX)
Call or Email for Price5 x HOT FIREPol® EvaGreen® HRM Mix is an optimised ready-to-use solution for High Resolution Melt (HRM) Analysis, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR and HRM Analysis: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol® DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of nonspecifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.
Learn More