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QPCR

MEDIBENA Life Science & Diagnostic Solutions

Probe Based Approach

Intercalating-Dye Approach

High-Resolution Melting (HRM)

FREE SAMPLES Solis BioDyne Product line Catalog  -


Convenient set-up at room temperature

As all Solis BioDyne products are stable at room temperature, reaction set-up does not require ice. This feature is especially valuable for users of high throughput platforms where keeping reaction conditions cold for hours is quite challenging. Using Solis BioDyne Mixes at room temperature will not affect the reaction performance. No loss of activity was detected after the mix was kept at 35°C for a month.

 

Light protective packaging

DNA intercalating dye EvaGreen® and reference dye ROX necessary in many qPCR mixes are sensitive to light and therefore require protective shielding. Solis packages all these qPCR mixes in special dark tubes to prevent harm from potential light exposure during transportation and reaction set-up.

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(Serial of tenfold dilutions of 72 bp fragment of Albumin gene was amplified from human genomic DNA. Reactions were performed on an Applied Biosystems 7900HT Real-Time PCR System following cycling protocols recommended by the suppliers.)


Higher precision and wider scale

All qPCR mixes from Solis BioDyne are produced as 5 x mixes that occupy only one fifth of the reaction final volume leaving 2.5 times more room for the template compared to 2 x mixes. This is highly advantageous when making wide-range serial dilutions or in case of gene expression studies where the concentration of the target gene is very low. Using the 5 x mix enables the  researcher to add more cDNA to the reaction resulting in better  precision and smaller Cycle threshold (Ct) values.


 


 

One mix for all cyclers requiring ROX

Compatibility with different cyclers requiring ROX Identical tenfold dilutions of 72 bp fragment of Albumin gene were simultaneously amplified with HOT FIREPol® Probe qPCR Mix Plus on three different platforms: Applied Biosystems 7500 Fast Real-Time PCR System (purple), Applied Biosystems StepOnePlus™ (green) and Applied Biosystems 7900HT Real-Time PCR System (pink).

  

 

Mix compatibility table

qPCR platforms

Probe based
approach

Intercalating dye based
approach

Applied Biosystems 7900HT, 7500, 7700, 7000,

StepOne™ & StepOnePlus™
Stratagene  
Mx3000P™ & Mx3005P™

5 x HOT FIREPol®
Probe qPCR  
Mix Plus (ROX)

5 x HOT FIREPol®
EvaGreen®
qPCR Mix Plus (ROX)

Bio-Rad CFX96™ & CFX384™, iQ™5 & MyiQ™,

Chromo4™, Opticon® 2 & MiniOpticon®;

Qiagen Rotor-Gene® Q, Rotor-Gene® 6000

Eppendorf Mastercycler® ep realplex2 & ep realplex4

5 x HOT FIREPol®
Probe qPCR
Mix Plus (no ROX)

5 x HOT FIREPol®
EvaGreen®
qPCR Mix Plus (no ROX)

Roche  LightCycler® 1.x, LightCycler® 2.0

5 x HOT FIREPol®
Probe qPCR
Mix Plus (Capillary)

5 x HOT FIREPol®
EvaGreen®
qPCR Mix Plus (Capillary)

Product Catalog -,   Price List -


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  1. SB_08-26-GP 5x HOT FIREPol EvaGreenĀ® qPCR Mix (Capillary)

    5x HOT FIREPol EvaGreenĀ® qPCR Mix (Capillary)

    Call or Email for Price

    HOT FIREPol® EvaGreen® qPCR Mix Plus (Capillary) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

    Learn More
  2. SB_04-33-GP 5x HOT FIREPolĀ® GC Master Mix TESTING SAMPLE

    5x HOT FIREPol GC Master Mix

    Call or Email for Price

    5x HOT FIREPol GC Master Mix is designed to provide highly specific high-yield amplification of GC-rich templates. Master Mix is a ready-to-use solution containing all reagents required for PCR (except template, primers and water). 100% DMSO and 25 mM MgCl2 are included in a package in separated vials. Generated PCR products are compatible with TA cloning procedures Master Mix for Highly specific high-yield amplification of GC-rich templates. Compatible with TA cloning. Learn More
  3. SB_08-25-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus (no ROX)

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus (no ROX)

    Call or Email for Price

    HOT FIREPol® EvaGreen® qPCR Mix Plus (no ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2 and EvaGreen® dye. The user simply needs to add water, template and primers. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

    Learn More
  4. SB_08-24-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus with ROX

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Mix Plus with ROX

    Call or Email for Price

    HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX) is an optimised ready-to-use solution for real-time quantitative PCR assays, incorporating EvaGreen® dye. It comprises all the components necessary to perform qPCR: HOT FIREPol® DNA Polymerase, ultrapure dNTPs, MgCl2, EvaGreen® dye and ROX dye.

    Learn More
  5. SB_08-36-GP 5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix

    Call or Email for Price

    5x HOT FIREPolĀ® EvaGreenĀ® qPCR Supermix is an optimised ready-to-use solution for real time quantitative PCR assays, incorporating EvaGreenĀ® dye. Suitable for ROX-dependent and ROX-independent qPCR cyclers Learn More
  6. SB_08-17-GP 5x HOT FIREPolĀ® Probe Universal qPCR Mix

    5x HOT FIREPolĀ® Probe Universal qPCR Mix

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    Suitable for ROX-dependent and ROX-independent qPCR cyclers Learn More
  7. SB_06-13-GP FIREScript Reverse Transkriptase (2 vials) FIREScript KIT

    FIREScript Reverse Transkriptase (2 vials) FIREScript KIT

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    FIREScript RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RTā€qPCR (quantitative reverse transcription PCR).

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  8. SB_06-15-GP FIREScript RT cDNA Synthesis KIT (7 vials)

    FIREScript RT cDNA Synthesis KIT (7 vials)

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    FIREScript RT cDNA synthesis Kit contains 7 reagents for ready to use Reverse Tascription. Learn More
  9. SB_06-Mix-GP FIREScript RT cDNA synthesis MIX (3 vials)

    FIREScript RT cDNA synthesis MIX (3 vials)

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    FIREScript RT cDNA synthesis Mix (3 vials) Learn More
  10. BO_370501 Hot DuroTaq ā„¢ Probe qPCR Mix (ROX Plus)

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