Convenient set-up at room temperature

As all Solis BioDyne products are stable at room temperature, reaction set-up does not require ice. This feature is especially valuable for users of high throughput platforms where keeping reaction conditions cold for hours is quite challenging. Using Solis BioDyne Mixes at room temperature will not affect the reaction performance. No loss of activity was detected after the mix was kept at 35°C for a month.

Light protective packaging

DNA intercalating dye EvaGreen® and reference dye ROX necessary in many qPCR mixes are sensitive to light and therefore require protective shielding. Solis packages all these qPCR mixes in special dark tubes to prevent harm from potential light exposure during transportation and reaction set-up.

(Serial of tenfold dilutions of 72 bp fragment of Albumin gene was amplified from human genomic DNA. Reactions were performed on an Applied Biosystems 7900HT Real-Time PCR System following cycling protocols recommended by the suppliers.)

Higher precision and wider scale

All qPCR mixes from Solis BioDyne are produced as 5 x mixes that occupy only one fifth of the reaction final volume leaving 2.5 times more room for the template compared to 2 x mixes. This is highly advantageous when making wide-range serial dilutions or in case of gene expression studies where the concentration of the target gene is very low. Using the 5 x mix enables the  researcher to add more cDNA to the reaction resulting in better  precision and smaller Cycle threshold (Ct) values.

One mix for all cyclers requiring ROX

Compatibility with different cyclers requiring ROX Identical tenfold dilutions of 72 bp fragment of Albumin gene were simultaneously amplified with HOT FIREPol® Probe qPCR Mix Plus on three different platforms: Applied Biosystems 7500 Fast Real-Time PCR System (purple), Applied Biosystems StepOnePlus™ (green) and Applied Biosystems 7900HT Real-Time PCR System (pink).

qPCR platforms

Probe based
approach

Intercalating dye based
approach

Applied Biosystems 7900HT, 7500, 7700, 7000,

StepOne™ & StepOnePlus™
Stratagene  Mx3000P™ & Mx3005P™

5 x HOT FIREPol®
Probe qPCR  
Mix Plus (ROX)

5 x HOT FIREPol®
EvaGreen®
qPCR Mix Plus (ROX)

Bio-Rad CFX96™ & CFX384™, iQ™5 & MyiQ™,

Chromo4™, Opticon® 2 & MiniOpticon®;

Qiagen Rotor-Gene® Q, Rotor-Gene® 6000

Eppendorf Mastercycler® ep realplex² & ep realplex⁴

5 x HOT FIREPol®
Probe qPCR
Mix Plus (no ROX)

5 x HOT FIREPol®
EvaGreen®
qPCR Mix Plus (no ROX)

Roche  LightCycler® 1.x, LightCycler® 2.0

5 x HOT FIREPol®
Probe qPCR
Mix Plus (Capillary)

5 x HOT FIREPol®
EvaGreen®
qPCR Mix Plus (Capillary)