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PCR and Hot Start PCR Polymerase

FIREPol® DNA Polymerase

FIREPol® is highly processive, thermostable and robust Taq polymerase with amplification range up to 3 kb. Due to Solis Tag technology, FIREPol® has an enhanced stability at ambient temperature with no activity loss up to 1 month.
We supply FIREPol® Polymerase as enzyme kit with a selection of buffers and an enhancer for personal optimization.

Hot FIREPol® DNA Polymerase

Hot Start PCR prevents misextension from non-specifically annealed primers and primer-dimers. HOT FIREPol®  DNA Polymerase is a chemically modified FIREPol®  DNA Polymerase. At ambient temperature it is inactive having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 12-15 min incubation step at 95º C. Hot-start master mixes are also available in ready-to-load format with variety of MgCl2 concentrations. Polymerases are stable at ambient temperature for up to one month. This makes handling and set-up even more convenient, since no ice has to be used.

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  1. SB_01-01-GP FIREPol® DNA Polymerase (5U/ul)

    FIREPol® DNA Polymerase (5U/ul)

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    FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

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  2. SB_01-02-GP HOT FIREPol® (HOT Start DNA Polymerase)

    HOT FIREPol® (HOT Start DNA Polymerase)

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    HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase. At ambient temperatures is inactive, having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 15min incubation step at 95°C. This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

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  3. SB_02-21-GP dNTP

    100mM dNTP Set (dATP, dCTP, dGTP and dTTP)

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    The dNTP Set contains of 100 mM solutions of dATP, dCTP, dGTP and dTTP each in separate vials in TE buffer. Learn More
  4. SB_02-31-GP dNTP

    80mM dNTP Mix (20mM each)

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    This ready-to-use mix consists of dATP, dCTP, dGTP and dTTP (each at a final concentration of 20 mM) in TE buffer, within one vial. The total concentration is 80 mM. Learn More
  5. SB_06-13-GP FIREScript Reverse Transkriptase (2 vials) FIREScript KIT

    FIREScript Reverse Transkriptase (2 vials) FIREScript KIT

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    FIREScript RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

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  6. SB_06-15-GP FIREScript RT cDNA Synthesis KIT (7 vials)

    FIREScript RT cDNA Synthesis KIT (7 vials)

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    FIREScript RT cDNA synthesis Kit contains 7 reagents for ready to use Reverse Tascription. Learn More
  7. SB_06-Mix-GP FIREScript RT cDNA synthesis MIX (3 vials)

    FIREScript RT cDNA synthesis MIX (3 vials)

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    FIREScript RT cDNA synthesis Mix (3 vials) Learn More

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MEDIBENA Life Science & Diagnostic Solutions | Zeillergasse 38 (Office: Paschinggasse 8), 1170 Vienna, Austria, Europe | office@medibena.at | TEL: +43-199-064-97 | FAX: +43-1-2533-0339-577 | Skype: medibena | Register of companies: Handelsgericht Wien Österreich | FN: 330781v | VAT: ATU65121744 | EORI: ATEOS1000017901 | Medibena Dr. Bencur Peter E.U. | www.MEDIBENA.com