FIREPol® is a highly processive, thermostable DNA polymerase. Due to its genetic modifications FIREPol® has an enhanced stability at room temperature with no activity loss for up to 1 month. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

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HOT FIREPol® DNA Polymerase is a chemically modified FIREPol® DNA Polymerase. At ambient temperatures is inactive, having no polymerization activity. HOT FIREPol® DNA Polymerase is activated by a 15min incubation step at 95°C. This prevents extentension of non-specifically annealed primers and primer-dimers formed at low temperatures during PCR setup. The enzyme has 5’→3’ polymerization-dependent exonuclease replacement activity but lacks 3’→ 5’ exonuclease activity.

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The dNTP Set contains of 100 mM solutions of dATP, dCTP, dGTP and dTTP each in separate vials in TE buffer. Learn More

This ready-to-use mix consists of dATP, dCTP, dGTP and dTTP (each at a final concentration of 20 mM) in TE buffer, within one vial. The total concentration is 80 mM. Learn More

10x GC - rich Enhancer is used as PCR additive for difficult GC - rich templates. The optimized solution modifies melting behavio u r of nucleic acids and often enhances amplification of suboptimal PCR systems with high degree of seco ndary structures and GC - rich regions. 10x GC - rich Enhancer should be used at a defined working concentration (1x, 2x or 3x solution) and only if non - specific amplifi cation occurs. Learn More

FIREScript RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).

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FIREScript RT cDNA synthesis Kit contains 7 reagents for ready to use Reverse Tascription. Learn More