Description

 The Tissue Total RNA Purification Mini Kit is designed for purification of total RNA from tissue using the chaotropic salt-lysis method without the use of hazardous solvents such as phenol. The Kit can quickly purify total RNA from up to 10mg of tissues within 30 minutes. The purified RNA is suitable for direct use in RT-PCR, Northern blotting, primer extension and cDNA library construction.

Features

•  High Purity: OD260/280: 1.9-2.1
•  High Speed: Rapid speed for the purification high quality RNA from samples ranging in size from 10mg of tissues in about 20~30 minutes, depending on the amount of starting material.
•  SafeUse: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials.

Time Required

About 20~30 minutes .
Applications
•  Nothern blotting hybridizations
•  Primer extension
•  RT-PCR
•  RNase protection assays
•  Differential display

•  As starting material for purification of mRNA for cDNA synthesis

Sample Size

Up to 20mg Tissue

Procedure

The method uses detergents and a chaotropic salt to lysis cell and inactivate RNase, then RNA in chaotropic salt is bonded to the glass fiber matrix of column. After washing off the contaminants, the purified RNA is eluted by RNase-free water. The entire procedure can be completed about 20~30 minute.

Sample

Recomended amount of sample used

RNA Yield(μg)