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Total RNA Extraction

MEDIBENA Life Science & Diagnostic Solutions

 

Tissue Total RNA Mini / Midi / Maxi Kit

 

 
 Total RNA isolation     

Description

 The Tissue Total RNA Purification Mini Kit is designed for purification of total RNA from tissue using the chaotropic salt-lysis method without the use of hazardous solvents such as phenol. The Kit can quickly purify total RNA from up to 10mg of tissues within 30 minutes. The purified RNA is suitable for direct use in RT-PCR, Northern blotting, primer extension and cDNA library construction.

Features

•  High Purity: OD260/280: 1.9-2.1
•  High Speed: Rapid speed for the purification high quality RNA from samples ranging in size from 10mg of tissues in about 20~30 minutes, depending on the amount of starting material.
•  SafeUse: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous materials.

Time Required

About 20~30 minutes .
Applications
•  Nothern blotting hybridizations
•  Primer extension
•  RT-PCR
•  RNase protection assays
•  Differential display

•  As starting material for purification of mRNA for cDNA synthesis

Sample Size

Up to 20mg Tissue

Procedure

The method uses detergents and a chaotropic salt to lysis cell and inactivate RNase, then RNA in chaotropic salt is bonded to the glass fiber matrix of column. After washing off the contaminants, the purified RNA is eluted by RNase-free water. The entire procedure can be completed about 20~30 minute.


 

Yield of Purification

Sample

Recomended amount of sample used

RNA Yield(μg)

Animal cells (up to 5x106) NIH/3T3 1x106 cells 10

HeLa 1x106 cells 15

COS-7 1x106 cells 30

LMH 1x106 cells 12
Animal Tissues Mouse/Rat, (up to 30mg)
Embryo 10 mg 25

Liver 10 mg 50

Brain 10 mg 10

Lung 10 mg 10

Heart 10 mg 10

Kidney 10 mg 30

Spleen 10 mg 35

Thymus 10 mg 45
Bacterial E.coli 1x10E9 cells 60

B.subtilis 1x10E9 cells 40
Yeast (up to 5x107) S.cerevisiae 1x10E7 cells 25

Storage Conditions

FavorPrep™ Tissue Total RNA Mini Kit can be stored at room temperature (15-25 ℃ ). Stable for 1 year at room temperature at 15-25℃.

Products

Cat. No. Product Name prep
  FATRK 001 Tissue Total RNA Mini Kit (50prep) 50
  FATRK 001-1 Tissue Total RNA Mini Kit (100prep) 100
  FATRK 001-2 Tissue Total RNA Mini Kit (300prep) 300
  FATRK 003 Tissue Total RNA Maxi Kit (10prep) 10
  FATRK 003-1 Tissue Total RNA Maxi Kit (24prep) 24

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  1. FABRK 001-GP Blood/Cultured Cell Total RNA Mini Kit

    Blood/Cultured Cell Total RNA Mini Kit

    Call or Email for Price

    The Blood/Cultured Cell Total RNA Purification Mini Kit is designed for purification of total RNA from cultured cells and fresh whole blood using the chaotropic salt-lysis method without the use of hazardous solvents such as phenol. The Kit can quickly purify total RNA from up to 300μl of blood within 30 minutes. The purified RNA is suitable for direct use in RT-PCR, Northern blotting, primer extension and cDNA library construction. Learn More
  2. FATRK 001-GP Tissue Total RNA Extraction Mini Kit

    Tissue Total RNA Mini Kit

    Call or Email for Price

    The Tissue Total RNA Purification Mini Kit is designed for purification of total RNA from tissue using the chaotropic salt-lysis method without the use of hazardous solvents such as phenol. The Kit can quickly purify total RNA from up to 10mg of tissues within 30 minutes. The purified RNA is suitable for direct use in RT-PCR, Northern blotting, primer extension and cDNA library construction. Learn More

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MEDIBENA Life Science & Diagnostic Solutions | Zeillergasse 38, 1170 Vienna, Austria, Europe | office@medibena.at | TEL: +43-699-1133-0065 | FAX: +43-1-2533-0339-577 | Skype: medibena | Register of companies: Handelsgericht Wien Österreich | FN: 392173a | VAT: ATU67954225 | EORI: ATEOS1000086649 | Dr. Monika Bencurova MEDIBENA Life Science E.U. | www.MEDIBENA.at