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MEDIBENA Life Science & Diagnostic Solutions

FavorPrep™ Plasmid DNA Extraction

  Mini Kit
  Midi / MAXI Kit
  Filter Kit
  MEGA Kit

  

    

 


Plasmid DNA Extraction Mini     GEL/PCR Purification Mini Kit

Description

The Plasmid Extraction Kit provide a rapid, phenol-free method for the extraction of high-purity plasmid DNA from bacterial cultures such as E. coli, which bacteria is pelleted, lysed, and then neutralized. The extracted DNA can be used in a variety of applications such as PCR, cloning, sequencing, in vitro transcription, and labeling. Also, as a column-type tube is utilized in the purification process, extraction is carried out in three simple steps of binding/washing/elution. Once bound, the DNA is washed and then eluted from the column, ready for use.

Features

•  For high yields of plasmid DNA-up to 50μg from 1~5ml overnight cultures.
•  Effective purification of DNA fragments ranging from 100bp to 12+kb.
•  No need for messy resin slurries, extracting with phenol, or concentrating via alcohol precipitation.
•  Superior purity-DNA yields quality sequence data using automated or manual methods.
•  Optimized buffers are included for maximum DNA purity and yield.
•  Versatile protocol-works with all neutral gel buffers and both conventional and low-melting agarose gel.

Time Required

Under 30 minutes

DNA Sequencing analysis of FavorPrep Plasmid DNA Extration

DNA Sequencing analysis of FavorPrep Plasmid DNA Extraction Kit Apparatus: ABIPRISM 3730 Protocols: DYEnamic ET terminator cycles sequencing ready Reaction Kit: FavorPrep Plasmid DNA Extraction Kit Template: pGEM-T


   

 

DNA Sequencing analysis

Apparatus: ABIPRISM 3730
Protocols: DYEnamic™ ET terminator cycles sequencing ready
Reaction Kit: FavorPrep Plasmid DNA Extraction Kit
Template: pGEM

Applications

•  Sequencing
•  Ligation
•  Restriction enzyme digestion
•  Transformation

Restriction analysis of 4 plasmids extracted by FavorPrep Plasmid DNA
Extration Kit. Digestion with EcoRI.

Agarose Gel Analysis Of Purified Plasmids. Restriction analysis of 4 plasmids extracted by FavorPrep Plasmid DNA Extration Kit. Digestion with EcoRI.

   

 

Agarose Gel Analysis Of Purified Plasmids


M: 1kb DNA ladder (100bp-12,000bp)
1: pCAMBIA 1304 cloned with 1,600bp EoRI insert (13kb)
2. pBluescript (2.9kb)
3: pF1 (11kb)
4: pR1 (5.3kb)

Storage Conditions

FavorPrep™ Plasmid DNA Extraction Kit can be stored at room temperature (15-25 ℃ ). After adding RNase A, PDE Buffer1, should be stored at 4 ℃ and is stable for six months. Other buffers and columns can be stored dry for up to 1 year at room temperature (15-25 ℃ ).

Plasmid DNA Miniprep Comparison

Additional Information

Cat. No.

Product Name

Size

Kit Contents

Store at

FAPDE 001

FavorPrep™ Plasmid DNA Extraction Kit

100 preps.

1. PDE Buffer 1 25ml
2. PDE Buffer 2 25ml
3. PED Buffer 3 45ml
4. Wash Buffer1 45ml
5. Wash Buffer 2 12.5ml
6. Elution Buffer 6ml
7. RNase (100 mg/ml) 50μl
8. PDE Column 100 preps
9. Collection Tube 100 tubes

PDE Buffer with RNaseA at 4 ℃ , others at room temperature.

FAPDE 001-1

FavorPrep™ Plasmid DNA Extraction Kit

300 preps.

1. PDE Buffer 1 65ml
2. PDE Buffer 2 75ml
3. PED Buffer 3 100ml
4. Wash Buffer 1 130ml
5. Wash Buffer (conc.)50ml
6. Elution Buffer 30ml
7. RNase (100 mg/ml) 130μl
8. PDE Column 300 preps
9. Collection Tube 300 tubes

PDE Buffer with RNaseA at 4 ℃ , others at room temperature.

 

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  1. FAPDE-mini-GP FavorPrepâ„¢ Plasmid Extraction Mini Kit

    Plasmid Extraction Mini Kit

    Call or Email for Price

    Plasmid Extraction Mini Kit is an excellent tool offering a speed and economic method to purify plasmid DNA from bacteria cultures. This technology is based on binding DNA to silica-based membranes in chaotropic salts, washing DNA with ethanol-contained Wash Buffer. Compare with other harmful and time-consuming procodure, such as phenol/ chloroform extraction and ethanol precipitation, Plasmid extraction kit shortens the handling time to about 25 minutes. The high quality plasmid DNA can be used directly for the downstream application. Learn More
  2. GPD-GP GEL/PCR Purification Mini Kit

    GEL/PCR Purification Mini Kit

    Call or Email for Price

    Gel/PCR Purification Kit is a cost effective tool that gives you a quick purification where yields are not important. IT is a dual purpose kit designed to recover or concentrate DNA Fragments (70bp-12Kb) from agarose gel or clean PCR or other enzymatic reaction.

    Learn More

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MEDIBENA Life Science & Diagnostic Solutions | Zeillergasse 38, 1170 Vienna, Austria, Europe | office@medibena.at | TEL: +43-699-1133-0065 | FAX: +43-1-2533-0339-577 | Skype: medibena | Register of companies: Handelsgericht Wien Österreich | FN: 392173a | VAT: ATU67954225 | EORI: ATEOS1000086649 | Dr. Monika Bencurova MEDIBENA Life Science E.U. | www.MEDIBENA.at