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MAXI, Midi

MEDIBENA Life Science & Diagnostic Solutions

FavorPrep™ Plasmid DNA Extraction

  Mini Kit
  Midi / MAXI Kit
  Filter Kit
  MEGA Kit

  

    

 


Plasmid Extraction Midi/Maxi Kit

 

Description

 The Plasmid Extraction Maxi Kit provides pre-packed an ion exchange rasin column for high-purity plasmid DNA maxiperp from 50-300ml bacterial cultures. Depending on the copy-number of plasmids, either a 50-150ml (high copy number) or a 150-400ml (low-copy plasmid) bacterial suspension could be used in the process. When using the standard protocol, the entire midiprep process can be completed in 120 minutes or less and up to 1000 μg of plasmid can be expected. The purity of purified plasmid DNA is equity to that obtained by 2x CsCl-gradient centrifugation and suitable for all molecular biological applications.

Features

 •  Purity: equity to that obtained by 2x CsCl-gradient centrifugation .
•  Safe: Eliminates the use of phenol, chloroform, ethidium bromide, and cesium chloride, minimizing exposure to, and disposal of hazardous  materials.
•  Time saving: Complete the process in less than 120 minutes.

          

Applications

 The purified plasmid DNA can be immediately used in any downstream molecular biology application.


•  Transfecttion
•  Microinjection
•  Sequencing
•  PCR
•  Restriction enzyme digestion


Operation Format

 Gravity-Flow

Time Required

 Less than 120 minutes

 

 

Agarose Gel Analysis Of Plasmids Purified by FavorPrep Midi/Maxi Kit

Agarose Gel Analysis Of Plasmids Purified with Favorgen Plasmid DNA Midi/Maxi Kit

 

 

Plasmid pBluescript (high-copy) and pBR322 (low-copy) are purified with the Plasmid DNA Midi/Maxi Kit. DNA in crude lysate from alkaline lysis and fractions from each gravity-flow step was collected by isopropanol precipitation and load to each lane.

C: crude lysate, F: flow-ghrough, W: wash, E: elute, M: 1Kb DNA Ladder

 

Recommended Culture Volume

Expected Yield
  High-copy plasmid Low-copy plasmid  
Midi
  25-50ml 50-150ml up to 400μg
MAXI
  50-150ml 150-500ml up to 1000μg



      

 

Procedure

In the process, the modified alkaline lysis method and RNase treatment are used to get cleared cell lysate plasmid DNA in crude lysate has been bound to the column, the contaminants can be washed off with wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in 120minutes without ultracentrifuges and HPLC or other toxic reagents.

Applications

 • Transfecttion
•  Microinjection
•  Restriction enzyme digestion
•  Sequencing
•  PCR 

Storage Conditions

Plasmid DNA Extraction Kit can be stored at room temperature (15-25 ℃ ). After adding RNase A, PDE Buffer1, should be stored at 4 ℃ and is stable for six months. Other buffers and columns can be stored dry for up to 1 year at room temperature (15-25 ℃ ).

 

Cat. No. Product Name Size Kit Contents Store at
FAPDE 002 Plasmid DNA Extraction Midi Kit 25 preps. 1. PM1 Buffer
2. PM2 Buffer
3. PM3 Buffer
4. PEQ Buffer
5. PW Buffer
6. PEL Buffer
7. RNase A (50mg/ml)
8. PI Midi columns (White filter)
110ml
110ml
110ml
130ml
240+120ml
220ml
200μl
25ea
PDE Buffer with RNaseA at 4 ℃ , others at room temperature.
FAPDE 003 Plasmid DNA Extraction Maxi Kit 10 preps. 1. PM1 Buffer
2. PM2 Buffer
3. PM3 Buffer
4. PEQ Buffer
5. PW Buffer
6. PEL Buffer
7. RNase A (50mg/ml)
8. PM Midi columns (White filter)
110ml
110ml
110ml
130ml
240+120ml
130ml
200μl
10ea
PDE Buffer with RNaseA at 4 ℃ , others at room temperature.

 

 


Recommended Culture Volume

Expected Yield

High-copy plasmid

Low-copy plasmid


Midi 25-50ml

50-150ml

up to 400μg

Maxi 50-150ml

150-500ml

up to 1000μg

 

Applications
•  Transfecttion
•  Microinjection
•  Restriction enzyme digestion
•  Sequencing
•  PCR

2 Item(s)

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Set Descending Direction
  1. FAFTE-MAXI-GP FavorPrepâ„¢ FavorFilterâ„¢ Plasmid Extraction MAXI Kit

    Plasmid Extraction MAXI Kit

    Call or Email for Price

    The Filter Plasmid DNA Extraction Maxi Kit is designed for rapid and efficient extraction of Ultra high quality plasmid DNA, with large-scale up to 1000 ug production of endotoxin-free plasmids for successful In Cell expression experiments. Learn More
  2. FAPDE-MAXI-GP Plasmid DNA ExtractionMaxi Kit, Ion Exchange, EndoToxinFree

    Plasmid Extraction MAXI Kit (+EndoToxinFree)

    Call or Email for Price

    The Plasmid Extraction Maxi Kit provides pre-packed an ion exchange rasin column for high-purity plasmid DNA maxiperp from 50-300ml bacterial cultures and up to 1000 ug of plasmid can be expected. With alternative of EndoToxin Free preparation. Learn More

2 Item(s)

per page

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MEDIBENA Life Science & Diagnostic Solutions | Zeillergasse 38, 1170 Vienna, Austria, Europe | office@medibena.at | TEL: +43-699-1133-0065 | FAX: +43-1-2533-0339-577 | Skype: medibena | Register of companies: Handelsgericht Wien Österreich | FN: 392173a | VAT: ATU67954225 | EORI: ATEOS1000086649 | Dr. Monika Bencurova MEDIBENA Life Science E.U. | www.MEDIBENA.at