MAXI, Midi
| Mini Kit Midi / MAXI Kit Filter Kit MEGA Kit |
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Plasmid Extraction Midi/Maxi Kit
Description
The Plasmid Extraction Maxi Kit provides pre-packed an ion exchange rasin column for high-purity plasmid DNA maxiperp from 50-300ml bacterial cultures. Depending on the copy-number of plasmids, either a 50-150ml (high copy number) or a 150-400ml (low-copy plasmid) bacterial suspension could be used in the process. When using the standard protocol, the entire midiprep process can be completed in 120 minutes or less and up to 1000 μg of plasmid can be expected. The purity of purified plasmid DNA is equity to that obtained by 2x CsCl-gradient centrifugation and suitable for all molecular biological applications.
Features • Purity: equity to that obtained by 2x CsCl-gradient centrifugation . |
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ApplicationsThe purified plasmid DNA can be immediately used in any downstream molecular biology application.
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Operation Format
Gravity-Flow |
Time RequiredLess than 120 minutes |
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Agarose Gel Analysis Of Plasmids Purified by FavorPrep Midi/Maxi Kit
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Plasmid pBluescript (high-copy) and pBR322 (low-copy) are purified with the Plasmid DNA Midi/Maxi Kit. DNA in crude lysate from alkaline lysis and fractions from each gravity-flow step was collected by isopropanol precipitation and load to each lane. C: crude lysate, F: flow-ghrough, W: wash, E: elute, M: 1Kb DNA Ladder |
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Recommended Culture Volume |
Expected Yield | ||
| High-copy plasmid | Low-copy plasmid | ||
| Midi |
25-50ml | 50-150ml | up to 400μg |
| MAXI |
50-150ml | 150-500ml | up to 1000μg |
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ProcedureIn the process, the modified alkaline lysis method and RNase treatment are used to get cleared cell lysate plasmid DNA in crude lysate has been bound to the column, the contaminants can be washed off with wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in 120minutes without ultracentrifuges and HPLC or other toxic reagents. Applications • Transfecttion Storage ConditionsPlasmid DNA Extraction Kit can be stored at room temperature (15-25 ℃ ). After adding RNase A, PDE Buffer1, should be stored at 4 ℃ and is stable for six months. Other buffers and columns can be stored dry for up to 1 year at room temperature (15-25 ℃ ). |
| Cat. No. | Product Name | Size | Kit Contents | Store at | |
| FAPDE 002 | Plasmid DNA Extraction Midi Kit | 25 preps. | 1. PM1 Buffer 2. PM2 Buffer 3. PM3 Buffer 4. PEQ Buffer 5. PW Buffer 6. PEL Buffer 7. RNase A (50mg/ml) 8. PI Midi columns (White filter) |
110ml 110ml 110ml 130ml 240+120ml 220ml 200μl 25ea |
PDE Buffer with RNaseA at 4 ℃ , others at room temperature. |
| FAPDE 003 | Plasmid DNA Extraction Maxi Kit | 10 preps. | 1. PM1 Buffer 2. PM2 Buffer 3. PM3 Buffer 4. PEQ Buffer 5. PW Buffer 6. PEL Buffer 7. RNase A (50mg/ml) 8. PM Midi columns (White filter) |
110ml 110ml 110ml 130ml 240+120ml 130ml 200μl 10ea |
PDE Buffer with RNaseA at 4 ℃ , others at room temperature. |
Recommended Culture Volume |
Expected Yield |
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High-copy plasmid |
Low-copy plasmid |
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Midi 25-50ml |
50-150ml |
up to 400μg |
Maxi 50-150ml |
150-500ml |
up to 1000μg |
Applications
• Transfecttion
• Microinjection
• Restriction enzyme digestion
• Sequencing
• PCR
2 Item(s)
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FAFTE-MAXI-GP
Plasmid Extraction MAXI Kit
Call or Email for PriceThe Filter Plasmid DNA Extraction Maxi Kit is designed for rapid and efficient extraction of Ultra high quality plasmid DNA, with large-scale up to 1000 ug production of endotoxin-free plasmids for successful In Cell expression experiments. Learn More -
FAPDE-MAXI-GP
Plasmid Extraction MAXI Kit (+EndoToxinFree)
Call or Email for PriceThe Plasmid Extraction Maxi Kit provides pre-packed an ion exchange rasin column for high-purity plasmid DNA maxiperp from 50-300ml bacterial cultures and up to 1000 ug of plasmid can be expected. With alternative of EndoToxin Free preparation. Learn More
2 Item(s)