NEXTflex™ ChIP-Seq Kit

The primary benefits of these kits include: 

• Faster protocol - bead-based cleanup protocol shaves hours off of time required for library prep
• Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
• Flexible barcode options - kits containing 6, 12, 24, 48 and 96 unique barcodes

• Low 10 ng input requirement
• For use with ChIP or genomic DNA samples
• Faster protocol - bead-based cleanup protocol shaves hours off of time required for library prep
• Enhanced Adapter Ligation Technology offers a larger number of unique sequencing reads
• 96 barcoded adapters for multiplexing contain embedded index sequence
• Automation-friendly workflow is compatible with liquid handlers
• Flexible barcode options – kits containing 6, 12, 24, 48 and 96 unique barcodes
• Compatible with Illumina GAIIx, HiSeq 2000 and MiSeq sequencing platforms

The NEXTflex™ ChIP-Seq Kit contains specially designed enzymes and buffers needed for library preparation of chromatin immunoprecipitated (ChIP) DNA or small genomic DNA samples for next generation sequencing. The NEXTflex ChIP-Seq Kit features Bioo Scientific’s proprietary “Enhanced Adapter Ligation Technology” resulting in library preps with a larger number of unique sequencing reads. By making improvements to the ligation enzymatic mix, the user will now have the ability to perform ligations with longer adapters and expect to see better binding efficiencies. The NEXTflex™ ChIP-Seq Kit simplifies workflow by using master mixed reagents and magnetic bead based cleanup, reducing pipetting and eliminating time consuming steps in library preparation. Using the Enhanced Adapter Ligation Technology with NEXTflex™ ChIP-Seq Barcodes, the user can index up to 96 samples at once providing an unparalleled ChIP sequencing capacity.

ChIP-Seq allows the examination of DNA sequences bound by proteins in cells. After using an antibody to enrich for a specific DNA-protein complex, the NEXTflex™ ChIP-Seq Kit can be used to prepare a library for cluster generation.