MagnetoFectionâ„¢

Magnetic nanoparticles for nucleic acids and small molecules delivery

• This method associates nucleic acids or other vectors with magnetic nanoparticles coated with cationic molecules. The magnetic nanoparticles are made of iron oxyde, which is fully biodegradable, coated with specific cationic proprietary molecules varying upon applications. Their association with the gene vectors (DNA, siRNA, ODN, virus, etc.) is achieved by salt-induced colloidal aggregation and electrostatic interaction.

Intracellular uptake of nanoparticles

The resulting molecular complexes are then targeted to and endocyted by cells, supported by an appropriate magnetic field. Membrane architecture and structure stay intact in contrast to other physical transfection methods that damage, create hole or electroshock the cell membranes. In addition the magnetic nanobeads are completely biodegradable and not toxic at the recommended doses and even higher. In this manner, the complete applied vector dose gets concentrated on the cells within a few minutes. Consequently, 100% of the cells get in contact with a significant vector dose.

Intracellular release of vectors and drugs         

The nucleic acids or other vectors are then released into the cytoplasm by different mechanisms depending on the formulation used. One is the proton sponge effect caused by cationic polymers coated on the nanoparticles that promotes endosome osmotic swelling, disruption of the endosomal membrane and intracellular release of associated molecules. Second is the destabilization of the endosome by cationic lipids coated on the particles that release the vectors into cells by flip-flop of cell negative lipids and charged neutralization. Third one is the usual viral mechanism when virus is used.

Inspired by the validated and recognized magnetic drug targeting technology, this innovative method is a revolution for transfection and infection.

Several optimized reagents have been designed according to define applications:

• Magnetofectamine™   

Combination of CombiMag and Lipofectamine™2000 from Invitrogen™ to increase trasfection efficiency.

• Viro-MICST™

Magnetic cell purification and transduction all-in-one

• PolyMag Neo

A new reagent for all nucleic acids transfection

• CombiMag

for enhancing all transfection reagents efficiency

• ViroMag and ViroMag R/L

for virus transduction

• AdenoMag

for adenovirus & AAV transduction  NEW

• SilenceMag

for siRNA applications

• Neuromag

for Neurons transfection