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5x HOT FIREPol® EvaGreen® qPCR Supermix

Availability: In stock

Product Name Price Qty
5x HOT FIREPol EvaGreen® qPCR Supermix - 0,02ml (25rxn) TEST SAMPLE - Ref.:SB_08-36-0000S Request Price
5x HOT FIREPol EvaGreen® qPCR Supermix - 250 rxn (1ml) - Ref.:SB_08-36-00001
€ 57,60
5x HOT FIREPol EvaGreen® qPCR Supermix - 2000 rxn (8ml) - Ref.:SB_08-36-00008
€ 451,20
5x HOT FIREPol EvaGreen® qPCR Supermix - 5000 rxn (20ml) - Ref.:SB_08-36-00020
€ 1.008,00
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Quick Overview

5x HOT FIREPol® EvaGreen® qPCR Supermix is an optimised ready-to-use solution for real time quantitative PCR assays, incorporating EvaGreen® dye. Suitable for ROX-dependent and ROX-independent qPCR cyclers

5x HOT FIREPol® EvaGreen® qPCR Supermix

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Additional Information

Cat. No. SB_08-36-GP
Manufacturer Solis BioDyne
Benefits Highly specific and reproducible real time PCR Excellent efficiency in case of low copy number targets UNG treatment capability due to dNTP blend of dUTP/dTTP Superior performance with long (up to 500 bp) and GC-rich templates Blue visualisation dye for easy pipetting

5x HOT FIREPol® EvaGreen® qPCR Supermix comprises all the components necessary, excluding the template and primers, to perform highly sensitive qPCR.
HOT FIREPol® DNA Polymerase is activated by a 12 min incubation step at 95°C. Hot-start mechanism prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

Detection and quantification of DNA and cDNA targets
Profiling gene expression 
Microbial detection
Viral load determination
5x HOT FIREPol® EvaGreen® qPCR Supermix is designed for use with standard cycling mode on standard and fast qPCR platforms regardless of requirements in ROX. The Mix is compatible with:
Applied BioSystems: QuantStudio™ 12K Flex, ViiA™ 7, 7900HT, 7500, 7700, StepOneTM & StepOnePlus™
Stratagene: MX3000P™ , MX3005P™
Bio-Rad: CFX96™ & CFX384™, iQ™5 & MyiQ™,  Chromo4™, Opticon® 2 & MiniOpticon® 
Qiagen: Rotor-Gene® Q, Rotor-Gene® 6000 Eppendorf: Mastercycler®: ep realplex2 & ep realplex4
Illumina: The Eco™
Roche: LightCycler® 480
Mix Composition
HOT FIREPol® DNA Polymerase Optimized buffer
12.5 mM MgCl2
1x PCR solution – 2.5 mM MgCl2
dNTP blend containing dUTP/dTTP EvaGreen® dye
Internal reference based on ROX dye GC-enhancer
Blue visualisation dye
Shipping and Storage Conditions
Routine storage: -20ºC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® EvaGreen® qPCR SuperMix.

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