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sb_08-14-GP

HOT FIREPol® Probe qPCR Mix Plus (ROX)

Availability: In stock

Product Name Price Qty
5x HOT FIREPol Probe qPCR Mix Plus (ROX) - 0,1ml (25rxn) TEST SAMPLE - Ref.:SB_08-14-0000S Request Price
5x HOT FIREPol Probe qPCR Mix Plus (ROX) - 1ml (250rxn) - Ref.:SB_08-14-00001
€ 68,00
5x HOT FIREPol Probe qPCR Mix Plus (ROX) - 8ml (2000rxn) - Ref.:SB_08-14-00008
€ 512,00
5x HOT FIREPol Probe qPCR Mix Plus (ROX) - 20ml (5000rxn) - Ref.:SB_08-14-00020
€ 1.224,00
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Quick Overview

HOT FIREPol®Probe qPCR Mix Plus (ROX) is optimized for real-time quantitative PCR assays and contains all the components necessary to perform qPCR, with the exception of template, primers, and probe. The qPCR Mix contains optimized components and HOT FIREPol® DNA Polymerase supplied in a proprietary reaction buffer that enables detection of low copy number targets. HOT FIREPol®DNA Polymerase is activated by a 15 min incubation step at 95°C. This prevents extension of non-specifically annealed primers and primer-dimers formed at low temperatures during qPCR setup.

HOT FIREPol® Probe qPCR Mix Plus (ROX)

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Additional Information

Cat. No. sb_08-14-GP
Manufacturer Solis BioDyne
Link to

Product Catalog,   Price List

Description

 

Applications:

- Detection and quantification of DNA and cDNA targets
- Profiling gene expression
- Microbial detection
- Viral load determination

Mix Composition:

- HOT FIREPol® DNA Polymerase
- 5 x Probe qPCR buffer- 15 mM MgCl
2, 1 x PCR solution – 3 mM MgCl2
- dNTPs
, including dTTP to improve reaction sensitivity and efficiency compared to dUTP
- ROX dye

ROX Dye:

ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.

Mix Compatibility Table

Applied Biosystems

7900HT, 7500, 7700, 7000,

StepOne™ & StepOnePlus™

Stratagene

Mx3000P™ & Mx3005P™

MEDIBENA Life Science & Diagnostic Solutions

 

Related products
ProductPack sizeCat. No.
 5 x HOT FIREPol® Probe qPCR Mix Plus (no ROX)
 1 ml  08-15-00001
 5 x HOT FIREPol® EvaGreen® qPCR Mix Plus (ROX)
 1 ml  08-24-00001
Key Features Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT FIREPol® Probe qPCR Mix Plus (ROX).
Details Applications: • Detection and quantification of DNA and cDNA targets • Profiling gene expression • Microbial detection • Viral load determination Mix Composition: • HOT FIREPol® DNA Polymerase • 5 x Probe qPCR buffer • 15 mM MgCl2 1 x PCR solution – 3 mM MgCl2 • dNTPs, including dTTP to improve reaction sensitivity and efficiency compared to dUTP • ROX dye ROX Dye: ROX is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR.
Notes Safety warnings and precautions: This product and its components should be handled only by persons trained in laboratory techniques. It is advisable to wear suitable protective clothing, such as laboratory overalls, gloves and safety glasses. Care should be taken to avoid contact with skin or eyes. In case of contact with skin or eyes, wash immediately with water. Some applications this product is used in may require a license which is not provided by the purchase of
Storage Temp -20°C
Shipping Conditions Ambient

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