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HBM-DNAc-GP

EXO-DNA Circulating and EV associated DNA extraction kit

Availability: In stock

Product Name Price Qty
EXO-DNAc Kit - plasma and serum (20rxn) - Ref.:HBM-DNAc-PS-20 Request Price
EXO-DNAc Kit - plasma and serum (40rxn) - Ref.:HBM-DNAc-PS-40 Request Price
EXO-DNAc Kit - urine and cell culture media (40rxn) - Ref.:HBM-DNAc-C-40 Request Price
EXO-DNAc Kit - urine and cell culture media (20rxn) - Ref.:HBM-DNAc-C-20 Request Price
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Quick Overview

Efficient platform for DNA mutation discovery by qPCR and digital PCR. DNA suitable for NGS

EXO-DNA Circulating and EV associated DNA extraction kit

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  • EXO-DNAc Kit - Circulating and EVs associated DNA

Additional Information

Cat. No. HBM-DNAc-GP
Manufacturer HansaBioMed LLC
Description

Circulating and EVs associated DNA

Circulating and EVs associated DNA Circulating DNA is emerging as a novel non-invasive tool for patient’s stratification and disease monitoring. While most of the research has focused on circulating cell-free (cfDNA) or circulating-tumor- cell-(CTC)-derived DNA, extracellular vesicle-(EVs)-associated DNA (EV-DNA) is emerging as a third valuable “liquid biopsy” platform. In fact genomic single or double-stranded DNA and mitocondrial DNA have been recently detected in extracellular vesicles. In particular the majority of double-stranded DNA seems to be associated with tumor derived exosomes (Thakur BK et al. 2014; Kalhert et al. 2014 ) where it represents the entire genome of the cancerous tumor from which exosomes were derived. This discovery corroborates the potential of exosomes, which can be easily obtained from a simple blood test.

Get genomic DNA with EXO-DNAc Kit

EXO-DNAc Kit combines the ability of our reagent DNA-Prep to co-isolate EVs and circulating DNA from biofluids (plasma, urine, serum etc..) or culture supernatants with a user friendly system for DNA purification. Isolated vesicles are lysed with the appropriate lysis buffer and DNA is purified by spin columns and optimized buffers with a fast turnaround time (approximatively 30 minutes). A. Finally, EXO-DNAc provides an appropriated DNA concentrator for concentrating the yield (4 fold concentration) and increasing the purity of the DNA to the levels required for digital PCR analysis.

Key Features

Applications
• Discovering of mutations by qPCR and digital PCR
• Direct exosome capture and DNA purification from biofluids of cell media without time consuming purification steps.
• Isolation and profiling of genomic EV-associated DNA by DNAse treatment.
• DNA suitable for NGS

Advantages
• Highly pure circulating and EVs-associated DNA
• Fast and easy protocol
• Small volume amount required
• Suitable for concomitant profiling of cell-free circulating DNA and EV-associated DNA

Storage Temp See Notes
Shipping Conditions See Notes

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