OZ_TN3000-GP

3D-FectIN™

Availability: In stock

Product Name	Price	Qty
3D-FectIN™ sample 25µL - Ref.:OZ_TN30025	€ 15,00
3D-FectIN™ 250µL (65 to 125 transfections with 1µg of DNA) - Ref.:OZ_TN30250	€ 180,00
3D-FectIN™ 500µL (125 to 250 transfections with 1µg of DNA) - Ref.:OZ_TN30500	€ 258,00
3D-FectIN™ 1mL (250 to 500 transfections with 1µg of DNA ) - Ref.:OZ_TN31000	€ 480,00

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Quick Overview

3D-FectIN™ is the newest reagent specifically developed to directly transfect cells cultured in 3D hydrogel. 3D-FectIN™ is suitable for all kind of hydrogels and cells. This reagent is based on a novel technology that allows adding a third dimension to cell culture.

Data Sheet -

Material Safety Data Sheet (MSDS)-

3D-FectIN™

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Additional Information

Cat. No.

OZ_TN3000-GP

Quantity / Size

100-1000ul

Manufacturer

OZ Biosciences

Link to

File Icon 3D-FectIN protocol, 3D-FectIN results, MSDS 3D-FectIN

Benefits

Highly efficient: on cell lines and primary cells
Specific for 3D hydrogels
All type of nucleic acids: plasmid DNA, siRNA, shRNA...
Completely biodegradable
Serum compatible
Long term protein expression

Description

3D Transfection: a novel perspective for your cells !

3D matrices not only add a third dimension to cells’ environment, they also allow creating significant differences in cellular characteristics and behavior. Because 3D matrices are routinely used in basic research and therapeutic applications, we have developed 3D-FectIN™ transfection reagent specific for hydrogel. In this way, hydrogel-based 3D matrices combined with 3D-FectIN™/DNA complexes, are colonized by cells to be transfected in a more natural environment. This method allows studying angiogenesis, tube and acini formation, colonization, neurite growth, tissue engineering, tissue regeneration, tumor invasion, neural differentiation, cellular polarization, tissue formation…

Procedure Overview

High transfection efficiency in various cells

Transfection of various cells on different gels

3D-FectIN™ has been successfully tested on a variety of cells.

Transfection efficiency and transgene expression achieved are high and long-lasting.

High secreted alkaline phosphatase expression

Efficient with various type of hydrogels and matrices

Name	Type of Scaffold
Collagen	Collagen-based hydrogels
Collagen-derived	Collagen-derived hydrogels
HA	Hyaluronic acid
Gelatin	Extracellular Matrix (ECM)
Fibrin / Fibronectin	ECM
Fibrinogen	ECM
Laminin	ECM
Matrigel™ *	BD Bioscience
Poly-(Ethylene Glycol)	PEGylated hydrogels

*Matrigel™ is a trademark own by BD Bioscience

Outperforms competitors

Comparison of RFP expression in HEK-293 cells

Comparison of secreted alkaline phosphatase production in NIH-3T3 cells

Product listing

TN30025

3D-FectIN™ sample

TN30250	3D-FectIN™ 250µL
	65 to 125 transfections with 1µg of DNA

TN30500	3D-FectIN™ 500µL
	125 to 250 transfections with 1µg of DNA

TN31000	3D-FectIN™ 1mL
	250 to 500 transfections with 1µg of DNA

Storage Temp

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Shipping Conditions

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